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ibicuruzwa

Magnetic Isaro ya ADN Ikuramo Ibikoresho Byihuta Ikusanyamakuru rya ADN

Ibisobanuro bigufi:

CY-F006-10 (50preps selile)

CYF006-11 (100preps-selile)

CY-F006-12 (200preps-selile)

CY-F006-20 (50preps-amacandwe)

CY-F006-21 (100preps-amacandwe)

CY-F006-22 (200preps-amacandwe)

Ibipimo byo gupakira:

Abantu 50 / agasanduku, abantu 100 / agasanduku, abantu 200 / agasanduku


Ibicuruzwa birambuye

Ibicuruzwa

Kumenyekanisha ibicuruzwa:

Ibikoresho byo gukuramo ADN ya Iclean bitanga uburyo bwihuse kandi bunoze bwo gukoresha amasaro ya magnetiki yo kweza no gukuramo ADN (harimo na ADN ya genomic, mitochondrial na virusi ADN) mumyanya yabitswe, amacandwe, amazi yumubiri, hamwe na buccal, cervical, selile selile, selile bagiteri nibindi.

Urugero rwibinyabuzima Urugero rushobora kubikwa muri bffer yacu yihariye yo kubungabunga iminsi igera kuri 30 ku bushyuhe bwicyumba mbere yo gutunganywa nta gihombo kigaragara cyatanzwe mu musaruro wa ADN cyangwa mu bwiza (days iminsi 30 iyo ubitswe mu buryo bukonje)

ADN yo mu rwego rwo hejuru irashobora kwezwa mu minota 15 hatabayeho gukuramo fenol / chloroform cyangwa imvura igwa, hamwe na ADN igereranije umusaruro wa 8 μg kuri buccal swab.ADN isukuye, hamwe na 20-30 kb, irakwiriye kumurongo wo hasi nka PCR cyangwa izindi reaction zidasanzwe

Ibyiza byibicuruzwa

Gukora neza cyane, gukuramo ADN imwe yihariye, gukuraho cyane poroteyine zanduye hamwe nibindi binyabuzima kama selile.Ibice bya ADN byakuweho ni binini, bifite isuku nyinshi, bihamye kandi byizewe mu bwiza.

1. Ikoreshwa rya magnetiki rishingiye ku masaro yo gutandukanya ADN genomic idakeneye imiti ishobora guteza akaga, centrifugation, cyangwa vacuum manifolds, fenol na etanol.

2.Gusukura neza kandi neza ADN ya genomic muri buccal swabs yabantu muminota itarenze 15 ikurikira gutegura sample na lysis.

3.Lisisi yoroshye hamwe na Proteinase K idakeneye lysis ya mashini.

4. Kwanduza gake na RNA.

5. ADN ya genomic isukuye yerekana imikorere myiza yo hasi mubikorwa harimo PCR.

6. Harimo igikoresho cyagenewe gutunganyirizwa mu buryo bwikora umubare munini wicyitegererezo mu byapa 96-byiza ukoresheje robot ikora amazi.

Imikoreshereze y'ibicuruzwa:

Kugirango usukure & kwigunga kwa ADN (harimo genomic, mitochondrial, bacterial, parasite & virusi ADN) biva mumyenda, amacandwe, amazi yumubiri, hamwe na buccal, cervical, selile yuruhu, selile bagiteri, tissue, swabs, CSF, amazi yumubiri, inkari zogejwe. .

Amabwiriza y'ibicuruzwa

1. Mbere yo gukoresha reagent ya acide nucleic

①.Hindura proteinase k solvent mumashanyarazi yumye arimo proteinase k hanyuma uvange neza.

②.Ongeramo 18ml na 42ml ya Ethanol yuzuye kuri CY3 na CY4 yicyitegererezo CY-F006-10 (50preps-selile) na CY-F006-20 (50preps-amacandwe), hanyuma uvange neza.

③.Ongeramo 36ml na 84ml etanol yuzuye kuri CY3 na CY4 yicyitegererezo CY-F006-11 (100preps-selile) na CY-F006-21 (100preps-amacandwe) hanyuma uvange neza.

 

2. Intambwe zo gukuramo Swab:

CollectionSwab yumye yumye, ongeramo 0,6ml CY1 yamazi, 10ul proteinase K, vanga neza, ubishyire muri incubator yumuyaga kuri dogere selisiyusi 65 hanyuma ushiremo iminota 30 (cyangwa gukusanya amazi: icyitegererezo cya centrifuge kirimo swab wongeyeho igisubizo cyo kubungabunga gishyizwe kuri 12000 rpm kumunota 1, Komeza imvura, ukureho ndengakamere. Ongeramo 0,6ml CY1 yamazi, 10ul proteinase k, vanga neza, ubishyire muri incubator yumuyaga kuri dogere selisiyusi 65 hanyuma ube muminota 30).

②.Kuraho swab na centrifuge kuri 12000rpm kuri 1min.

③.Kuramo supernatant yose kumuyoboro mushya wa centrifuge hanyuma ukore igerageza.

④.Ongeramo 0.25ml CY2 y'amazi, amasaro 10ul ya magneti * (kunyeganyezwa neza mbere yo kuyakoresha), vanga neza kuri 12min, ubishyire kuri magnetiki hanyuma ureke bihagarare 30, hanyuma unywe amazi.

⑤ Ongeramo 0,6ml y'amazi ya CY3, vanga neza kuri 3min, ubishyire kuri magnetiki hanyuma ureke bihagarare 30s kugirango unywe amazi.

⑥.Ongeramo 0,6ml y'amazi ya CY4, vanga kuri 3min, ubishyire kuri magnetiki hanyuma ureke bihagarare 30s, unywe amazi

⑦.Subiramo intambwe ②⑥

⑧.Kama kuminota 10-20min mubushyuhe bwicyumba, ongeramo 50ul CY5 yamazi kugirango uhanagurwe, vanga neza, ubishyire kumurongo wa magneti hanyuma ureke bihagarare 30, hanyuma wohereze amazi mumiyoboro mishya ya centrifuge.

⑨.Gupima OD

 

3. Intambwe yo gukuramo amacandwe

① Centrifuge saa 12000rpm kuri 1min hamwe n'amacandwe wongeyeho kuvanga kubungabunga

② Komeza imvura kandi ukureho ndengakamere

③.Ongeramo 0,6ml y'amazi ya CY1 na 10ul ya proteinase k kuri yo, vanga neza, hanyuma ubishyire muri incubator yo mu kirere kuri dogere selisiyusi 65 hanyuma ushiremo iminota 30.

④ Centrifuge saa 12000rpm kuri 1min, fata supernatant yose kumuyoboro mushya wa centrifuge, ongeramo amasaro ya magneti 10ul na 0.25ml CY2, vanga neza kuri 12min, ubishyire kumurongo wa magneti hanyuma ureke bihagarare 30s kugirango unywe amazi.

⑤ Ongeramo 0,6ml y'amazi ya CY3, vanga neza kuri 3min, ubishyire kuri magnetiki hanyuma ureke bihagarare 30s kugirango unywe amazi.

⑥.Ongeramo 0,6ml y'amazi ya CY4, vanga kuri 3min, ubishyire kuri magnetiki hanyuma ureke bihagarare 30s kugirango unywe amazi.

⑦.Subiramo intambwe ⑥

⑧.Kama mubushyuhe bwicyumba muminota 10-20, ongeramo 50ul ya CY5 yamazi kugirango uhanagurwe, vanga neza, ubishyire kuri magnetiki hanyuma ureke bihagarare 30, hanyuma wohereze amazi mumiyoboro mishya ya centrifuge.

⑨.Gupima OD

Icyitonderwa: Niba ukeneye gukuraho RNA, urashobora gutegura RNaseA 10mg / ml: solvent (10mM sodium acetate: pH5.0), ukayitekesha 15min, ugahindura pH 7.5 hamwe na Tris-Hcl, hanyuma ukabika kuri dogere selisiyusi 20.

Imiterere yo kubika nigihe cyo kwemeza

1. Ibicuruzwa bigomba gukoreshwa mubidukikije bifite isuku nisuku, birinda umwanda, kandi bifite ubushyuhe bukwiye.

2. Bika ubushyuhe bwicyumba.Proteinase K n'amasaro ya magnetique birashobora kubikwa kuri 2-8 ° C mugihe kirekire.

3. Ibicuruzwa byo kubika ubuzima: amezi 12

Icyitonderwa:

1. Iki gicuruzwa gikoreshwa gusa mugupima vitro.

2. Ibidukikije byo kubika hamwe nintambwe zo gukuramo bigomba gukurikiza byimazeyo amabwiriza ari mu gitabo.

3. Niba ubona ko amafaranga ari make cyane mugihe cyo kuyakuramo, urashobora kongera muburyo bukwiye urugero cyangwa kongera umubare wibyakuwe.

4. ADN yakuweho igomba kuba nshya kandi ikageragezwa mugihe.

Icyitonderwa: Ubu buryo bwo gutwara abantu bukoreshwa mugupima vitro, kandi ntibushobora gukoreshwa imbere cyangwa hanze mubantu cyangwa inyamaswa.Niba yamizwe, irashobora gutera ibintu bikomeye;birakaza amaso n'uruhu.Niba itabishaka kumeneka mumaso, kwoza amazi.Igomba guhumeka mugihe cyo kuyikoresha.

Intangiriro

Huachenyang (Shenzhen) Technology Co., Ltd kabuhariwe mu gukora ibicuruzwa biva mu muhogo, mu muhogo, mu kanwa, mu mazuru, mu nda, inkondo y'umura, sponge swabs, kwanduza virusi, ibisubizo byo kwirinda virusi.Ifite imbaraga zimwe muruganda.ibyiza

Dufite Imyaka irenga 12+ yuburambe bwo gukora mubikoresho byubuvuzi

HCY ifata ubuziranenge bwibicuruzwa nkibyingenzi mu iterambere ry’imishinga, yubahiriza umuyobozi w’ibicuruzwa byo mu rwego rwa mbere, serivisi zo mu rwego rwa mbere ”mu buryo bwose, akurikiza umwuka w’ibikorwa byo“ gushaka ukuri, guhanga udushya, ubumwe no gukora neza ” .HCY itegura inzira yose yumusaruro nogurisha ukurikije sisitemu yo gucunga ISO9001 na ISO13485, hamwe nibikorwa bihamye kandi bifite ireme.


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